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The "sequence segment" field is used for a fast sequence-based search of DisProt, which will give you a list of DisProt proteins with significant similarity to the query sequence.

Please enter the sequence (without the Fasta header) using the single-letter AA code.


To provide fast sequence similarity search we employed the Reverse Position-Specific BLAST program (RPS-BLAST) originally designed for conserved domain search (CD-search). Unlike PSI-BLAST, RPS-BLAST searches a query sequence against a database of pre-calculated sequence profiles and reports significant hits in a single pass. With this approach, the search is extremely fast since it compares the query sequence with a limited number of profiles corresponding to the DisProt proteins, but also very specific since the sequence comparison is based on the profile of the corresponding disordered protein.

Through PSI-BLAST search for each disordered protein in DisProt, we constructed its sequence profile against the non-redundant database, with BLOSUM62 as the scoring matrix, with gap penalties as 11/1, and with the E-value inclusion threshold set to 0.0005. The maximum number of iterations was limited to 3 in order to constrain the influence of potential false positives. A profile database was then saved for future RPS-BLAST searches. Given a query sequence and a user-specified E-value cutoff, the RPS-BLAST program is invoked to search against the profile database and the local alignments with E-values below the cutoff are returned to the user.


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